Search results for "Apoptotic body"
showing 4 items of 4 documents
U937 variant cells as a model of apoptosis without cell disintegration
2012
AbstractThe variant cell line U937V was originally identified by a higher sensitivity to the cytocidal action of tumor necrosis factor alpha (TNFα) than that of its reference cell line, U937. We noticed that a typical morphological feature of dying U937V cells was the lack of cellular disintegration, which contrasts to the formation of apoptotic bodies seen with dying U937 cells. We found that both TNFα, which induces the extrinsic apoptotic pathway, and etoposide (VP-16), which induces the intrinsic apoptotic pathway, stimulated U937V cell death without cell disintegration. In spite of the distinct morphological differences between the U937 and U937V cells, the basic molecular events of ap…
PPAR-γ Agonist GW1929 But Not Antagonist GW9662 Reduces TBBPA-Induced Neurotoxicity in Primary Neocortical Cells
2013
Tetrabromobisphenol A (2,2-bis(4-hydroxy-3,5-dibromophenyl)propane; TBBPA) is a widely used brominated flame retardant. TBBPA induces neuronal damage, but the mechanism by which this occurs is largely unknown. We studied the possible involvement of peroxisome proliferator-activated receptor gamma (PPAR-γ) in TBBPA-induced apoptosis and toxicity in mouse primary neuronal cell cultures. TBBPA enhanced both, caspase-3 activity and lactate dehydrogenase (LDH) release in neocortical cells after 6 and 24 h of exposition. These data were supported at the cellular level with Hoechst 33342 staining. Immunoblot analyses showed that, compared with control cells, 10 μM TBBPA decreased the expression of…
TBBPA causes neurotoxic and the apoptotic responses in cultured mouse hippocampal neurons in vitro
2015
Abstract Background Tetrabromobisphenol A (TBBPA) is a brominated flame retardant widely used in a variety of commercial and household products. TBBPA can become bioaccumulated in human body fluids, and also in different brain regions. The aim of the present study was to determine the viability and apoptosis of cultured mouse hippocampal neurons in vitro after exposure to TBBPA. Additionally, we examined the involvement of ROS generation in the effect of TBBPA. Methods Primary hippocampal neuron cultures were prepared from Swiss mouse embryos on day 17/18 of gestation. The cultures were treated with TBBPA at concentrations ranging from 1 nM to 100 μM for 30 min or 3, 6 or 24 h. To study apo…
Triclosan induces Fas receptor-dependent apoptosis in mouse neocortical neurons in vitro
2014
Triclosan (TCS) is a commonly used antimicrobial agent in personal care and sanitizing products, as well as in household items. Numerous studies have demonstrated the presence of TCS in various human tissues. Several studies have reported the accumulation of TCS in fish and human brain tissue. The aim of the present study was to investigate the effect of TCS on apoptosis in mouse neocortical neurons after 7 days of culture in vitro following 3, 6 and 24 h of exposure. To explore the mechanism underlying the effects of TCS in neurons, we studied the activation and protein expression of the Fas receptor (FasR) and caspase- 8, caspase-9 and caspase-3, as well as DNA fragmentation in TCS-treate…